Macrophages Isolation in Pig (Monocyte, Peritoneal, Alveolar and Bone-Marrow)
Protocol to collect and freeze macrophages from the four different compartments (peritoneum, lungs, blood, bone-marrow) from pigs.
PBMC are isolated from blood after Ficool separation. Bone-marrow cells are flushed out of the ribs. Peritoneal macrophages are isolated by peritoneal lavage with PBS. Lungs are collected and flushed in order to isolate alveolar macrophages.
Protocol keywords:
macrophages
pig
cryopreservation
isolation
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Protocol source name:
Macrophages Isolation in Pig (Monocyte, Peritoneal, Alveolar and Bone-Marrow)
Protocol provider name (your name):
rkapetanovic
Protocol provider contact email (your email) :
ronan.kapetanovic@roslin.ed.ac.uk
